流式通道校准(Calibration)质控微球-用于确定系统的灵敏度和线性度，有无光污染
2025-04-19The SPHERO™ Calibration Particles are designed for routine calibration of flow cytometers. By using these particles, the sensitivity and linearity of the system can be determined. The sensitivity of the instrument will help detect any dye or light contamination. The linearity of the instrument will determine if the logarithmic amps and PMTs are functioning properly.
SPHERO™ Rainbow Calibration Particles
- Contains a mixture of fluorophores that enable the Rainbow Calibration Particles to be excited at any wavelength from 365 nm to 650 nm
- Allows the calibration of the FITC, PE, PE-TR, PE-Cy5, and APC channels in the flow cytometer with the same set of particles
- Consists of set of several similar size particles with different fluorescence intensities in a convenient dropper bottle
- Can be stored in the freezer and reused later since they can withstand repeated freezing and thawing
| 货号 | 品名 | 浓度 | 粒径 | 规格 | 备注 |
| SPBY-RCP-05-5 | Rainbow Calibration Particles, 4 peaks | 107/mL | 0.4-0.6 µm | 5 mL | 校准FITC,PE,PE-TR,PE-Cy5及APC通道 |
| SPBY-RCP-20-5 | Rainbow Calibration Particles, 4 peaks | 107/mL | 1.8-2.2 µm | 5 mL | 校准FITC,PE,PE-TR,PE-Cy5及APC通道 |
| SPBY-RCP-30-5 (6 peaks) | Rainbow Calibration Particles, 6 peaks | 107/mL | 3.0-3.4 µm | 5 mL | 校准FITC,PE,PE-TR,PE-Cy5及APC通道 |
| SPBY-RCP-30-5A (8 peaks) | Rainbow Calibration Particles, 8 peaks | 107/mL | 3.0-3.4 µm | 5 mL | 校准FITC,PE,PE-TR,PE-Cy5及APC通道 |
| SPBY-RCP-30-20A | Rainbow Calibration Particles, 8 peaks | 107/mL | 3.0-3.4 µm | 20 mL | 校准FITC,PE,PE-TR,PE-Cy5及APC通道 |
| SPBY-RCP-30-5A-2 | Rainbow Calibration Particles, 2nd peak from the 8 peak set |
107/mL | 3.0-3.4 µm | 5 mL | 校准FITC,PE,PE-TR,PE-Cy5及APC通道 |
| SPBY-RCP-32-5 | Rainbow Calibration Particles, 6 peaks | 107/mL | 3.2 (+/- 0.1) µm | 5 mL | 校准FITC,PE,PE-TR,PE-Cy5及APC通道 |
| SPBY-RCP-35-5 | Rainbow Calibration Particles, 4 peaks | 107/mL | 3.5-4.0 µm | 5 mL | 校准FITC,PE,PE-TR,PE-Cy5及APC通道 |
| SPBY-RCP-60-5 | Rainbow Calibration Particles, 6 peaks | 107/mL | 6.0-6.4 µm | 5 mL | 校准FITC,PE,PE-TR,PE-Cy5及APC通道 |
| SPBY-RCP-60-20 | Rainbow Calibration Particles, 6 peaks | 107/mL | 6.0-6.4 µm | 20 mL | 校准FITC,PE,PE-TR,PE-Cy5及APC通道 |
| SPBY-RQC-30-5 | Rainbow QC Calibration Particles, 3 peaks | 2x107/mL | 3.0-3.4 µm | 5 mL | 校准FITC,PE,PE-TR,PE-Cy5及APC通道 |
SPHERO™ Rainbow Calibration Particles (Euroflow)
| 货号 | 品名 | 浓度 | 粒径 | 规格 | 备注 |
| SPBY-RCP-30-5A (Euroflow) | Rainbow Calibration Particles, 8 peaks, Lot EAL01 | 107/mL | 3.0-3.4 µm | 5 mL | 校准FITC,PE,PE-TR,PE-Cy5及APC通道 (欧洲流式标准) |
SPHERO™ Ultra Rainbow Calibration Particles
- Designed for routine calibration of the flow cytometer in all channels
- Contain a mixture of fluorophores that enable the optimization of linearity, sensitivity, and resolution of the flow cytometer
- Especially useful in the Far Red and Violet channels
| 货号 | 品名 | 浓度 | 粒径 | 规格 | 备注 |
| SPBY-URCP-38-2K | Ultra Rainbow Calibration Kit, 6 peaks(Blank, 2x106/mL), (Fluorescent Particles, 107/mL) |
107/mL after mixing | 3.8 µm (+/- 0.2 µm) | 1x3 mL,1x2 mL | 拓展到远红外和紫外通道的超级彩虹校准球 |
| SPBY-URCP-38-20K | Ultra Rainbow Calibration Kit, 6 peaks(Blank, 2x106/mL), (Fluorescent Particles, 107/mL) |
107/mL after mixing | 3.8 µm (+/- 0.2 µm) | 1x30 mL,1x20 mL | 拓展到远红外和紫外通道的超级彩虹校准球 |
| SPBY-URCP-50-2K | Ultra Rainbow Calibration Kit, 6 peaks(Blank 2x106/mL), (Fluorescent Particles 107/mL) |
107/mL after mixing | 5.0-5.4 µm | 1x3 mL,1x2 mL | 拓展到远红外和紫外通道的超级彩虹校准球 |
| SPBY-URCP01-30-10K | Ultra Rainbow Calibration Particle Kit, 3 peaks | 106/mL | 3.0-3.4 µm | 10x3 mL | 拓展到远红外和紫外通道的超级彩虹校准球 |
| SPBY-URCP-100-2 | Ultra Rainbow Calibration Particles, 6 peaks | 106/mL | 8.0-12.9 µm | 2 mL | 拓展到远红外和紫外通道的超级彩虹校准球 |
| SPBY-URCP-100-2H | Ultra Rainbow Calibration Particles, 9 peaks | 107/mL | 8.0-12.9 µm | 2 mL | 拓展到远红外和紫外通道的超级彩虹校准球 |
| SPBY-URCP-100-6K | Ultra Rainbow Calibration Kit, 6 peaks | 107/mL after mixing | 8.0-12.9 µm | 6x2 mL | 拓展到远红外和紫外通道的超级彩虹校准球 |
SPHERO™ Spectral Matching Calibration Particle Kits
- Surface labeled polystyrene particles with commonly used fluorophores
- Used to determine the linearity of the logarithmic amps and sensitivity of flow cytometers in specific channels
- Supplied as individual products for use in specific channels of flow cytometers
| 货号 | 品名 | 浓度 | 粒径 | 规格 | 备注 |
| SPBY-ECFP-F1-5K | FITC Easy Calibration Kit, 5 peaks | 2x106/mL | 3.0-3.4 µm | 5x1 mL | FITC通道校准球 |
| SPBY-ECFP-F2-5K | PE Easy Calibration Kit, 5 peaks | 2x106/mL | 3.0-3.4 µm | 5x1 mL | PE通道校准球 |
| SPBY-ECFP-F9-6K | GFP Easy Calibration Kit, 6 peaks | 2x106/mL | 3.0-3.4 µm | 6x1 mL | GFP通道校准球 |
SPHERO™ Rainbow Linear Calibration Particles
| 货号 | 品名 | 浓度 | 粒径 | 规格 |
| SPBY-RLP-30-5 | Rainbow Linear Calibration Particles (For use in Linear Scale) | 107/mL | 3.0-3.4 µm | 5 mL |
SPHERO™ APC Calibration Particles
| 货号 | 品名 | 浓度 | 粒径 | 规格 | 备注 |
| SPBY-ACP-30-5K | APC Calibration Particle Kit (6 peaks) | 107/mL | 3.0-3.4 µm | 2x5 mL | APC通道校准球 |
SPHERO™ IR Calibration Particles
| 货号 | 品名 | 浓度 | 粒径 | 规格 | 备注 |
| SPBY-ACP-30-5K | APC Calibration Particle Kit (6 peaks) | 107/mL | 3.0-3.4 µm | 2x5 mL | APC通道校准球 |
| SPBY-FCP-34800-6K | Fluorescent Calibration Particles, 800nm EM, 6 peaks | 107/mL | 3.5 µm (+/- 0.2 µm) | 6x1 mL | IR通道校准球 |