流式补偿微球-用于多色流式细胞仪分析的荧光补偿设置
2025-04-19SPHERO™ COMPtrol: Fluorescent Conjugate QC and Antibody Binding Compensation Particles
- Coated with Goat anti-Mouse Ig to bind to the fluorochrome-conjugated monoclonal antibodies used for cell staining
- Provide a method for the quality control of the fluorochrome-conjugated monoclonal antibodies
- Aid in setting proper compensation to reduce the cross-talk between the channels of multicolor flow cytometers.
| 货号 | 品名 | 浓度 | 粒径 | 规格 | 备注 |
| SPBY-CMIgP-08-2K | COMPtrol Kit, Goat anti-Mouse Ig (H&L) Coated Particles,2 populations (Negative & High) | 1.0x107/mL | 0.7-0.9 µm | 2x5 mL | 相当于invitrogen的OneComp eBeads™ |
| SPBY-CMIgP-30-2K | COMPtrol Kit, Goat anti-Mouse Ig (H&L) Coated Particles,2 populations (Negative & High in separate viales) | 1.0x107/mL | 3.0-3.4 µm | 2x5 mL | 相当于invitrogen的OneComp eBeads™ |
| SPBY-CMIgP-30-5 | COMPtrol Kit, Goat anti-Mouse Ig (H&L) Coated Particles,2 populations (Negative & High) | 1.0x107/mL | 3.0-3.4 µm | 5 mL | 相当于invitrogen的OneComp eBeads™ |
| SPBY-CMIgP-50-3K | COMPtrol Kit, Goat anti-Mouse Ig (H&L) Coated Particles,3 populations (Negative, Low, & High) | 2.5x106/mL | 5.0-5.9 µm | 3x5 mL | 相当于invitrogen的OneComp eBeads™ |
| SPBY-CMIgP-70-3K | COMPtrol Kit, Goat anti-Mouse Ig (H&L) Coated Particles,3 populations (Negative, Low, & High) | 2.5x106/mL | 7.0-7.9 µm | 3x5 mL | 相当于invitrogen的OneComp eBeads™ |
SPHERO™ EasyComp: Spectral Matching Particles
- Surface labeled polystyrene particles with commonly used fluorophores
- Used for setting compensation in a wide variety of channels for any flow cytometer
- Supplied as individual products or as kits for use in different models of flow cytometers
| 货号 | 品名 | 浓度 | 粒径 | 规格 | 备注 |
| SPBY-ECFP-B | EasyComp Fluorescent Particles, Blank | 107/mL | 3.0-3.4 µm | 5 mL | 荧光微球做补偿，无需抗体，操作简单 |
| SPBY-ECFP-F1 | EasyComp Fluorescent Particles, FITC | 107/mL | 3.0-3.4 µm | 1 mL | 荧光微球做补偿，无需抗体，操作简单 |
| SPBY-ECFP-F2 | EasyComp Fluorescent Particles, PE | 107/mL | 3.0-3.4 µm | 1 mL | 荧光微球做补偿，无需抗体，操作简单 |
| SPBY-ECFP-F3 | EasyComp Fluorescent Particles, PE-TR | 107/mL | 3.0-3.4 µm | 1 mL | 荧光微球做补偿，无需抗体，操作简单 |
| SPBY-ECFP-F4 | EasyComp Fluorescent Particles, PE-Cy5 | 107/mL | 3.0-3.4 µm | 1 mL | 荧光微球做补偿，无需抗体，操作简单 |
| SPBY-ECFP-F5 | EasyComp Fluorescent Particles, PE-Cy7 | 107/mL | 3.0-3.4 µm | 1 mL | 荧光微球做补偿，无需抗体，操作简单 |
| SPBY-ECFP-F6 | EasyComp Fluorescent Particles, APC | 107/mL | 3.0-3.4 µm | 1 mL | 荧光微球做补偿，无需抗体，操作简单 |
| SPBY-ECFP-F7 | EasyComp Fluorescent Particles, APC-Cy7 | 107/mL | 3.0-3.4 µm | 1 mL | 荧光微球做补偿，无需抗体，操作简单 |
| SPBY-ECFP-F8 | EasyComp Fluorescent Particles, PerCP | 107/mL | 3.0-3.4 µm | 1 mL | 荧光微球做补偿，无需抗体，操作简单 |
| SPBY-ECFP-F9 | EasyComp Fluorescent Particles, GFP | 107/mL | 3.0-3.4 µm | 1 mL | 荧光微球做补偿，无需抗体，操作简单 |
| SPBY-ECFP-K1 | EasyComp Fluorescent Particle Kit (Blank, FITC, PE & PE-Cy5), 4 vials | 107/mL | 3.0-3.4 µm | 1x5 mL,3x1 mL | 荧光微球做补偿，无需抗体，操作简单 |
| SPBY-ECFP-K2 | EasyComp Fluorescent Particle Kit (Blank, FITC, PE, PE-Cy5 & APC), 5 vials | 107/mL | 3.0-3.4 µm | 1x5 mL,4x1 mL | 荧光微球做补偿，无需抗体，操作简单 |
| SPBY-ECFP-K3 | EasyComp Fluorescent Particle Kit (Blank, FITC, PE, PE-TR & PE-Cy5), 5 vials | 107/mL | 3.0-3.4 µm | 1x5 mL,4x1 mL | 荧光微球做补偿，无需抗体，操作简单 |
| SPBY-ECFP-K4 | EasyComp Fluorescent Particle Kit (Blank, FITC, PE, PE-TR, PE-Cy5, PE-Cy7, APC & APC-Cy7), 8 vials |
107/mL | 3.0-3.4 µm | 1x5 mL,7x1 mL | 荧光微球做补偿，无需抗体，操作简单 |
Flow Cytometry Antibody Binding Beads
Single population Protein A or Protein G microspheres are suitable for labeling with conjugated antibodies from a range of hosts. When bound to your fluorecently labeled antibody, these microspheres may be used as single-population for removing spectral overlap of fluorochromes.
| 货号 | 品名 | 浓度 | 粒径 | 规格 | 备注 |
| 553 | Flow Cytometry Protein A Antibody Binding Beads | 2.0e+6 beads/mL | 7-9µm | 1 mL | 可与大多数荧光一抗结合 |
| 554 | Flow Cytometry Protein G Antibody Binding Beads | 2.0e+6 beads/mL | 7-9µm | 1 mL | 可与大多数荧光一抗结合 |
Simply Cellular® Compensation Standards
Polystyrene beads coated with secondary antibodies that allow you to attach your fluorophore-conjugated mouse, rat, or human antibody. Once bound, these microspheres may be used as single-population for adjusting flow cytometers to avoid spectral overlap.
| 货号 | 品名 | 浓度 | 粒径 | 规格 | 备注 |
| 550 | Simply Cellular® Compensation Standard (anti-Mouse IgG) | 2.0e+6 beads/mL | 7-9µm | 1 mL | 相当于invitrogen的OneComp eBeads™ |
| 556 | Simply Cellular® Compensation Standard - High (anti-Mouse IgG) | 2.0e+6 beads/mL | 7-9µm | 1 mL | 相当于invitrogen的OneComp eBeads™ |
| 551 | Simply Cellular® Compensation Standard (anti-Rat IgG) | 2.0e+6 beads/mL | 7-9µm | 1 mL | 相当于invitrogen的OneComp eBeads™ |
| 552 | Simply Cellular® Compensation Standard (anti-Human IgG) | 2.0e+6 beads/mL | 7-9µm | 1 mL | 相当于invitrogen的OneComp eBeads™ |
| 835 | Simply Cellular® anti-Mouse for Violet Laser (anti-Mouse IgG) | 2.0e+6 beads/mL | 5µm | 1 mL | 相当于invitrogen的OneComp eBeads™ |
Viability Dye Compensation Standard
Viability Dye Compensation Standards are suitable for labeling with LIVE / DEAD® stains or other amine-reactive dyes to generate compensation standards for flow cytometric analyses. Beads are not suitable for labeling with DNA stains such as propidium iodide, DAPI, or SYTOX™, and users should contact us for discussion if uncertain as to the compatibility of a specific dye or stain. Supplied at ~1×107 beads/mL.
| 货号 | 品名 | 浓度 | 粒径 | 规格 | 备注 |
| 450 | Viability Dye Compensation Standard, 4µm | 1 e+7 beads/mL | 4µm | 1 mL | LIVE/DEAD®补偿用 |
| 451 | Viability Dye Compensation Standard, 8µm | 1 e+7 beads/mL | 8µm | 1 mL | LIVE/DEAD®补偿用 |
FITC/PE Compensation Standard
The FITC / PE Compensation Standard includes four microsphere populations that are ~7-9 µm in diameter, including an Autofluor™ population, and single populations of surface-labeled beads with FITC, PE, and FITC / PE. The Autofluor population are unlabeled beads exhibit autofluorescence at approximately the same intensity as unlabeled lymphocytes.
| 货号 | 品名 | 浓度 | 粒径 | 规格 | 备注 |
| 820 | FITC/PE Compensation Standard (Autofluor™, FITC, PE, FITC/PE) | 2.0e+6 beads/mL | 7-9µm | 1 mL | 专用于FITC 和PE荧光补偿用 |